Identification of miRNA from Bouteloua gracilis, a drought tolerant grass, by deep sequencing and their in silico analysis

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• 作者：Perla Lucí ; a Ordó ; ñ ; ez-Baquera^a ; ^{plordonez@uach.mx} ; Everardo Gonzá ; lez-Rodrí ; guez^a ; ^{evegonzal@uach.mx} ; Gerardo Armando Aguado-Santacruz^b ; ^c ; ^{gaguado@prodigy.net.mx} ; Quintí ; n Rascó ; n-Cruz^d ; ^{qrascon@uach.mx} ; Ana Conesa^e ; ^f ; ^{aconesa@cipf.es} ; ^{aconesa@ufl.edu} ; Veró ; nica Moreno-Brito^g ; ^{vmoreno@uach.mx} ; Raquel Echavarria^h ; ^{rechavarria.z@conacyt.mx} ; Joel Dominguez-Viveros^a ; ^{joeldominguez@hotmail.com}
• 关键词：EST ; Expressed Sequence Tag ; SOAP ; Short Oligonucleotide Alignment Program ; MFE ; Minimum Folding Energies ; UPE ; unpaired energy ; Osa ; Oryza sativa ; Zma ; Zea mays ; Sbi ; Sorghum bicolor ; Ath ; Arabidopsis thaliana
• 刊名：Computational Biology and Chemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：66
• 期：Complete
• 页码：26-35
• 全文大小：813 K
• 卷排序：66

文摘

We identified 31 conserved miRNAs families and 53 putative novel miRNAs in B. gracilis. Two putative miRNA sequences were unique to B. gracilis and they had B. gracilis-specific ESTs as targets. Predicted miRNAs putatively regulate mRNA genes that are involved in biological processes such as metabolism, plant growth and development, and stress responses.