The pUltra plasmid series: A robust and flexible tool for fluorescent labeling of Enterobacteria
详细信息 查看全文
- 作者：Despoina A.I. Mavridoua ; 1 ; d.mavridou@imperial.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Diego Gonzalezb ; 1 ; Abigail Clementsa ; Kevin R. Fosterb
- 关键词：GFP ; green fluorescent protein ; RFP ; red fluorescent protein ; YFP ; yellow fluorescent protein ; CFP ; cyan fluorescent protein ; OD600 ; optical density A600 ; DMEM ; Dulbecco's Modified Eagle Media ; MOI ; multiplicity of infection ; EHEC ; enterohaemorrhagic Escherichia coli ; PFA ; para-formaldehyde
- 刊名：Plasmid
- 出版年：2016
- 出版时间：September-November 2016
- 年：2016
- 卷：87-88
- 期：Complete
- 页码：65-71
- 全文大小：1434 K
文摘
- •
The pUltra plasmids are designed for fluorescent labeling of Enterobacteria.
- •
pUltra vectors with two antibiotic resistances are available in four colors each.
- •
Labeling is efficient and uniform without any growth rate effects to the cells.
- •
Color distinction during microscopy and flow cytometry is entirely unambiguous.
- •
The plasmids can label enteric pathogens even in association with their host cells.