Interspecies somatic cell nuclear transfer in Asiatic cheetah using nuclei derived from post-mortem frozen tissue in absence of cryo-protectant and in vitro matured domestic cat oocytes

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• 作者：F. Moulavi^a ; S.M. Hosseini^a ; ^{smhosseini@royaninstitute.org} ; N. Tanhaie-Vash^a ; S. Ostadhosseini^a ; S.H. Hosseini^b ; ¹ ; M. Hajinasrollah^a ; M.H. Asghari^a ; H. Gourabi^c ; A. Shahverdi^d ; A.D. Vosough^e ; M.H. Nasr-Esfahani^a ; ^{mh.nasr-esfahani@royaninstitute.org}
• 关键词：Asiatic cheetah ; Somatic cell nuclear transfer ; Domestic cat
• 刊名：Theriogenology
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：197-203
• 全文大小：864 K
• 卷排序：90

文摘

Non-viable nuclei obtained from Asiatic cheetah kept at −20 °C post-mortem were used for iSCNT. Cheetah cloned embryos developed in vitro before arresting at the morula stage. Embryonic fragmentation and abnormal chromosome segregation were evidenced in Cheetah iSCNT embryos. Genomic disintegrity was reasoned as major cause of failed Cheetah iSCNT blastocyst development.