Characterisation of carbapenem-resistant Acinetobacter baumannii outbreak strains producing OXA-58 in Turkey
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- 作者：Canan Kulah ; Marlies J. Mooij ; Fusun Comert ; Elif Aktas ; Guven Celebi ; Nagihan Ozlu ; Martine C. Rijnsburger ; Paul H.M. Savelkoul
- 关键词：Acinetobacter baumannii ; Outbreak ; OXA-58 ; blaOXA-58 ; Oxacillinase ; Carbapenems
- 刊名：International Journal of Antimicrobial Agents
- 出版年：2010
- 出版时间：August 2010
- 年：2010
- 卷：36
- 期：2
- 页码：114-118
- 全文大小：196 K
文摘
The aim of this study was to characterise the molecular epidemiology and mechanisms of carbapenem resistance of nosocomial Acinetobacter baumannii isolates in a new university hospital in Turkey. A total of 145 carbapenem-resistant A. baumannii (CRAB) isolates were collected during the period 2003–2006. All isolates were typed by amplified fragment length polymorphism (AFLP) analysis. AFLP analysis showed three predominant clusters consisting of 72, 20 and 12 clinical strains as well as some smaller clusters and 23 unique strains. The three main clonal AFLP types corresponded to three major antibiotic susceptibility patterns. One environmental isolate was found related to the major outbreak clone. The reference type strains of European clones I, II and III were also typed by AFLP and analysed for clonal similarity. Polymerase chain reaction (PCR) analysis of different carbapenem resistance genes showed that strains from each of the three main clusters as well as 79 % of the remaining strains harboured the blaOXA-58 gene. No genes encoding the metallo-β-lactamases GIM-1, SIM-1, SPM-1, IMP-like and VIM-like or the oxacillinases OXA-24-like and OXA-23-like were detected. In conclusion, multiple clones of CRAB strains producing OXA-58-type oxacillinase were responsible for a sustained CRAB outbreak occurring in a hospital in Turkey. These isolates were not associated with A. baumannii strains of the major European clones I, II or III.