Analysis of monoclonal antibody oxidation by simple mixed mode chromatography
详细信息 查看全文
- 作者：Jorge Alexander Pavon ; Xiaojuan Li ; Steven Chico ; Umesh Kishnani ; Soundara Soundararajan ; Jason Cheung ; Huijuan Li ; Daisy Richardson ; Mohammed Shameem ; Xiaoyu Yang ; xiaoyu.yang@merck.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mixed mode chromatography ; Methionine ; Tryptophan ; Oxidation ; Monoclonal antibody
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：1431
- 期：Complete
- 页码：154-165
- 全文大小：1250 K
文摘
- •
A novel mixed mode chromatography method using the unique property of Sepax Zenix SEC-300MK column was developed to analyze mAb oxidized species as prepeaks.
- •
This method can be used to monitor either methionine-oxidized species induced by the common oxidants TBHP, or the tryptophan-oxidized species triggered by AAPH, or oxidized species by UV photo-irradiation.
- •
The resolved oxidized prepeaks were confirmed by LC–MS as oxidized antibody variants with a mass increase of 16 or 32 Da.
- •
This method has been successfully applied to monitor oxidation of IgG1, IgG2, and IgG4 samples.