LFS respirometry allowed characterizing immobilized SOB from a desulfurizing BTF.
SOB oxidized preferentially sulfide with a SOURmax of 49.2 mg DO g−1 VSS min−1.
A shrinking particle model was used to describe elemental sulfur oxidation.
Sulfide oxidation was completely inhibited at 25.6% of intracellular sulfur stored.
Thiosulfate and elemental sulfur were the intermediates produced during S2− oxidation.