Evaluation of urine for Leishmania infantum DNA detection by real-time quantitative PCR

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• 作者：Rô ; mulo Pessoa-e-Silva^a ; ^b ; ^{romulops12@gmail.com} ; Lays Adrianne Mendonç ; a Trajano-Silva^a ; ^{laystrajano@gmail.com} ; Maria Almerice Lopes da Silva^a ; ^{almerice@cpqam.fiocruz.br} ; Suê ; nia da Cunha Gonç ; alves-de-Albuquerque^a ; ^b ; ^{sueniaaa@hotmail.com} ; Tayná ; Correia de Goes^a ; ^{taynacgoes@hotmail.com} ; Rayana Carla Silva de Morais^a ; ^{rayanacarla_m@hotmail.com} ; Fá ; bio Lopes de Melo^a ; ^{fabio@cpqam.fiocruz.br} ; Milena de Paiva-Cavalcanti^a ; ^{mp@cpqam.fiocruz.br}
• 关键词：Visceral leishmaniasis ; Diagnosis ; Real-time quantitative PCR ; Urine
• 刊名：Journal of Microbiological Methods
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：131
• 期：Complete
• 页码：34-41
• 全文大小：771 K
• 卷排序：131

文摘

An optimized real-time qPCR assay was chosen for L. infantum DNA detection in urine samples. The chosen of the most adequate and feasible DNA extraction protocol was pivotal. The detection limit reached was 5 fg (~ 0.025 parasites) per μL of urine. Correlations with implementation of treatment were found. The assay indicated a good negative predictive value in urine samples from untreated suspected patients.