Analysis of Panax notoginseng metabolites in rat bile by liquid chromatography-quadrupole time-of-flight mass spectrometry with microdialysis sampling

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• 作者：Xiao-Dong Wen^a ; ^b ; ¹ ; Jie Yang^b ; ¹ ; Rong-Hua Ma^b ; Wen Gao^b ; Lian-Wen Qi^b ; Ping Li^b ; Brent A. Bauer^c ; Guang-Jian Du^a ; Zhiyu Zhang^a ; Jacqueline Somogyi^a ; Chong-Zhi Wang^a ; Chun-Su Yuan^a ; ^{CYuan@dacc.uchicago.edu}
• 关键词：Microdialysis sampling ; Liquid chromatography&ndash ; quadrupole time-of-flight mass spectrometry ; Panax notoginseng ; Ginsenosides ; Bile ; Metabolites
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2012
• 出版时间：1 May, 2012
• 年：2012
• 卷：895-896
• 期：Complete
• 页码：162-168
• 全文大小：857 K

文摘

A dynamic microdialysis sampling method with liquid chromatography-quadrupole time-of-flight mass spectrometry (Q-TOF-MS) was developed for rapid and sensitive analysis of the metabolite profile of Panax notoginseng extract (PNE) in rat bile. In vivo studies in male Sprague-Dawley rats were performed with microdialysis probes implanted into the bile duct before bile samples were collected from 0 to 12 h. Metabolites of PNE were identified using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. The mass accuracy of precursor and fragment ions was typically within 5 ppm of the theoretical values. We identified 7 compounds: 4 parent compounds (notoginsenoside R1, ginsenosides Rg1, Rb1, and Rd) and 3 metabolites (ginsenosides Rg2, Rh2, and compound K). Data from this study suggest that this microdialysis technique could be used in notoginseng saponin metabolic animal studies.