Rapid chemical profiling of saponins in the flower buds of Panax notoginseng by integrating MCI gel column chromatography and liquid chromatography/mass spectrometry analysis

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• 作者：Wen-Zhi Yang^a ; Tao Bo^b ; Shuai Ji^a ; Xue Qiao^a ; De-An Guo^a ; Min Ye^a ; ^{yemin@bjmu.edu.cn}
• 关键词：Flower buds of Panax notoginseng ; Liquid chromatography/mass spectrometry ; Notoginseng saponins ; Chemical profiling
• 刊名：Food Chemistry
• 出版年：2013
• 出版时间：15 August, 2013
• 年：2013
• 卷：139
• 期：1-4
• 页码：762-769
• 全文大小：1241 K

文摘

The flower buds of Panax notoginseng (Notoginseng flower, FBP) are used as the traditional Chinese medicine San-Qi-Hua. In this study, we conducted column chromatography fractionation and liquid chromatography/mass spectrometry (LC/MS) analysis to comprehensively profile bioactive notoginseng saponins (ginsenosides) in FBP. MCI gel column chromatography allowed separation and enrichment of minor saponins. Electrospray ionization tandem mass spectrometry of [M?H]^? and [M+Na]⁺ precursor ions of the saponins provided reliable structural information for the sapogenin, and sequence of sugar chains. Confirmed by high-accuracy Q-TOF analysis, 170 notoginseng saponins were characterized from FBP, and 91 of them were reported from Panax species for the first time. The new ginsenosides contain acyl groups on ¦Á-chain, malonyl group at 20-OH, or di-malonyl groups. This study also indicated that the flower buds of P. notoginseng contained more protopanaxadiol-type but less protopanaxatriol-type ginsenosides than the roots.