A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies

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• 作者：Rogé ; rio A. Depieri ; Cé ; lia G.T.J. Andrade ; Antô ; nio R. Panizzi
• 关键词：Pentatomidae ; Southern green stink bug ; Nezara viridula ; Mouthparts ; Microscopic methodology ; Cross-section ; EDTA ; Microwave irradiation
• 刊名：Micron
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：41
• 期：2
• 页码：169-171
• 全文大小：229 K

文摘

A new methodology has been developed to produce sections as thin as 5 μm of stink bug (Heteroptera: Pentatomidae) mouthparts for morphological studies. Heads of the southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae) were fixed on Bouin solution, and treated with EDTA (ethylenediamine tetraacetic acid 7 % ) in an ice bath within a microwave oven for 0.5, 1, 2, 3, 4, and 5 h. The chitin structures and labium soft parts were well preserved after 0.5 h EDTA treatment. Longer times of exposure to EDTA and microwave irradiation caused damage to mouthpart structures.