Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences
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- 作者：Meng Wang ; Hong-xia Zhao ; Long Wang ; Tao Wang ; Rui-wu Yang ; Xiao-li Wang ; Yong-hong Zhou ; Chun-bang Ding ; Li Zhang
- 关键词：cpDNA ; chloroplast DNA ; nrDNA ; nuclear ribosomal DNA ; ITS ; internal transcribed spacer ; PCR ; polymerase chain reaction ; K2P ; Kimura 2-Parameter ; NJ ; neighbor-joining ; BS ; bootstrap support ; CBOL ; Consortium for the Barcode of Life
- 刊名：Gene
- 出版年：2013
- 出版时间：10 October, 2013
- 年：2013
- 卷：528
- 期：2
- 页码：206-215
- 全文大小：353 K
文摘
An effective DNA marker for authenticating the genus Salvia was screened using seven DNA regions (rbcL, matK, trnL-F, and psbA-trnH from the chloroplast genome, and ITS, ITS1, and ITS2 from the nuclear genome) and three combinations (rbcL + matK, psbA-trnH + ITS1, and trnL-F + ITS1). The present study collected 232 sequences from 27 Salvia species through DNA sequencing and 77 sequences within the same taxa from the GenBank. The discriminatory capabilities of these regions were evaluated in terms of PCR amplification success, intraspecific and interspecific divergence, DNA barcoding gaps, and identification efficiency via a tree-based method. ITS1 was superior to the other marker for discriminating between species, with an accuracy of 81.48 % . The three combinations did not increase species discrimination. Finally, we found that ITS1 is a powerful barcode for identifying Salvia species, especially Salvia miltiorrhiza.