Addition of Lippia alba (Mill) N. E. Brown essential oil to the diet of the silver catfish: An analysis of growth, metabolic and blood parameters and the antioxidant response
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- 作者：Etiane M.H. Saccol ; Juliano Uczay ; Tanise S. P锚s ; Isabela A. Finamor ; Giovana M. Ourique ; Ana P.K. Riffel ; Denise Schmidt ; Braulio O. Caron ; Berta M. Heinzmann ; Susana F. Llesuy ; Rafael Lazzari ; Bernardo Baldisserotto ; Maria A. Pavanato
- 关键词：Rhamdia quelen ; Oxidative stress ; Antioxidant defenses ; Lipid peroxidation ; Volatile organic compounds
- 刊名：Aquaculture
- 出版年：5 December, 2013
- 年：2013
- 卷：416-417
- 期：Complete
- 页码：244-254
- 全文大小：675 K
文摘
The growth, metabolic and blood parameters and oxidative stress biomarkers effects of adding Lippia alba essential oil (EO) to the diet (0 = control, 0.25, 0.5, 1.0 or 2.0 mL EO per kg diet) of juvenile silver catfish were investigated for 60 days. Liver and muscle were used to measure the glucose, glycogen and lactate levels. Lipid peroxidation (LPO) was assessed as a marker of oxidative damage. Enzymatic antioxidants, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST), and a non-enzymatic antioxidant, non-protein thiols (NPSH), were also quantified in the brain, gills, liver, kidney and muscle. The diet containing L. alba EO did not influence the growth and blood parameters of these animals. However, it reduced glucose levels and increased glycogen reserves in the liver. The diet containing 1.0 mL EO per kg diet increased liver lactate levels. In the muscle, the diet containing 0.5 mL EO per kg diet increased the glycogen levels. The levels of LPO were measured by determining the thiobarbituric acid reactive substance assay and lipid hydroperoxides, in the gills, liver, kidney and muscle and were also reduced in animals receiving the EO-containing diet; however, no LPO effect was observed in the brain. In turn, the diets containing EO increased the activity of SOD (0.5, 1.0 and 2.0 mL EO per kg diet), CAT, GPx, and GST and the NPSH content in the brain; the SOD, GR (2.0 mL EO per kg diet) and GST (0.5, 1.0 and 2.0 mL EO per kg diet) in the gills; the CAT, GR (2.0 mL EO per kg diet) and NPSH levels in the liver; the CAT (0.25, 0.5 and 1.0 mL EO per kg diet), GR (1.0 and 2.0 mL EO per kg diet) and NPSH (0.5, 1.0 and 2.0 mL EO per kg diet) levels in the kidney; and the SOD, GPx (0.5, 1.0 and 2.0 mL EO per kg diet) and NPSH (2.0 mL EO per kg diet) levels in the muscle. Thus, despite not improving the growth or blood parameters, the addition of L. alba EO to the diet of silver catfish is recommended because it decreases LPO, increases the glycogen and lactate reserves and increases the tissue antioxidant response.