- 作者：Justyna Teresa Polit ; Iwona Ciereszko
- 关键词：6-DMAP ; 6-dimethylaminopurine ; INV ; invertase ; MPF ; M-phase promoting factor ; NBT ; nitroblue tetrazolium salt ; OA ; okadaic acid ; PCP1-2 ; principal control points ; PP1/PP2A ; specific protein phosphatases ; SPF ; S-phase promoting factor ; SuSy ; sucrose syntha
- 刊名：Journal of Plant Physiology
- 出版年：2012
- 出版时间：1 November, 2012
- 年：2012
- 卷：169
- 期：16
- 页码：1597-1606
- 全文大小：2284 K
Summary
Carbohydrate starvation of Vicia faba root meristems leads to readjustment of carbohydrate metabolism and blocks the cell cycle in two principal control points (PCP1/2). The cell cycle reactivation is possible after sucrose provision, although with a delay of about 12 h. During this period, the cells are sensitive to 6-dimethylaminopurine (6-DMAP) and okadaic acid (OA), inhibitors of protein kinases and phosphatases, respectively. The aim of the present study was to investigate whether those inhibitors are involved in inhibition of cell cycle revival through interference with the activities of two sucrose-cleaving enzymes: sucrose synthase (SuSy; EC 2.4.1.13) and invertase (INV; EC 3.2.1.26).In sugar-starved cells, the in situ activity of both enzymes decreased significantly. Following supplementation of root meristems with sugar, INV remained inactive, but SuSy activity increased. Despite the lack of INV activity, glucose was present in meristem cells, but its content was low in cells treated with OA. In the latter case, the size of plastids was reduced, they had less starch, and Golgi structures were affected. In sugar-starved cells, SuSy activity was induced more by exogenous sucrose than by glucose. The sucrose-induced activity was strongly inhibited by OA (less by 6-DMAP) at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities. The results indicate that prolongation of regeneration and a marked decrease in the number of cells resuming proliferation (observed in previous studies) and resulting from the action of inhibitors, are correlated with the process of SuSy activation at the beginning of regeneration from sugar starvation.