Biochemical and biophysical methodologies open the road for effective schistosomiasis therapy and vaccination

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• 作者：Rashika El Ridi^a ; ^{rashikaelridi@hotmail.com} ; Hatem Tallima^a ; ^b ; Federica Migliardo^c
• 关键词：SM ; sphingomyelin ; nSMase ; neutral sphingomyelinase ; ARA ; arachidonic acid ; IF ; indirect membrane immunofluorescence ; MBCD ; methyl-β-cyclodextrin ; PUFA ; polyunsaturated fatty acid ; EINS ; elastic incoherent neutron scattering ; QENS ; quasi-elastic neutron scattering ; PZQ ; praziquantel ; ESP ; excretory&ndash ; secretory products ; SG3PDH ; glyceraldehyde 3-phosphate dehydrogenase
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3613-3620
• 全文大小：797 K
• 卷排序：1861

文摘

Cholesterol and sphingomyelin sequester larval schistosome outer membrane antigens. Sphingomyelin protects schistosomes via tight hydrogen bond barrier formation. QENS and EINS measurements were performed to study schistosomes mobility and rigidity. The measurements revealed the different diffusion and flexibility properties of larvae and adult parasites. Activator of neutral sphingomyelinase, arachidonic acid, is a new schistosomicide. Larval excretory-secretory products were advocated as sole vaccine candidates.