A novel LC–MS/MS method for simultaneous determination of nine alkaloids in human plasma.
Highly sensitive, the LLOQs of 1.5 pg/ml for palmatine and jatrorrhizine, and 15 pg/ml for the others.
The gradient elution was adopted to overcome the matrix effect.
Pharmacokinetics of the nine alkaloids of Shuanghua Baihe tablets in human were reported firstly.