The importance of start codon of nosM in nosiheptide production

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• 作者：Lei JIANG ; Yan-Jiu XUE¹ ; Wei-Ying LIU ; Min MA ; Xu-Ri WU ; Shu-Zhen WANG ; ^{shuzhenwang@cpu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yi-Jun CHEN ; ^{yjchen@cpu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nosiheptide ; Start codon ; Leader peptide ; Biosynthesi
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：13
• 期：11
• 页码：854-860
• 全文大小：903 K

文摘

The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.