Mimicking respiratory phosphorylation using purified enzymes
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- 作者：Christoph von Ballmoosa ; 1 ; christoph.vonballmoos@dcb.unibe.ch" class="auth_mail" title="E-mail the corresponding author ; Olivier Binera ; 2 ; Tobias Nilssonb ; 2 ; Peter Brzezinskib
- 关键词：FCCP ; carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone ; CCCP ; carbonyl cyanide m-chlorophenyl hydrazone ; Q1 ; ubiquinone Q1 ; ACMA ; 9-amino-6-chloro-2-methoxyacridine ; cytc ; cytochrome c
- 刊名：Biochimica et Biophysica Acta (BBA) - Bioenergetics
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1857
- 期：4
- 页码：321-331
- 全文大小：1024 K
文摘
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The functional co-reconstitution of terminal oxidases and ATP synthase is described.
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The experimental setup is used with enzymes from different organisms.
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Steady state ATP synthesis at high rates is observed.
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Mild uncoupling is not a protein dependent process.