Ceramide signaling targets the PP2A-like protein phosphatase Sit4p to impair vacuolar function, vesicular trafficking and autophagy in Isc1p deficient cells
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- 作者：Vitor Teixeiraa ; b ; c ; Tâ ; nia C. Medeirosb ; Rita Vilaç ; aa ; b ; Joã ; o Ferreirab ; Pedro Moradas-Ferreiraa ; b ; c ; Ví ; tor Costaa ; b ; c ; vcosta@ibmc.up.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：CAPP ; Ceramide-activated Protein Phosphatase ; CLS ; chronological lifespan ; CPY ; carboxypeptidase Y ; Cvt ; cytoplasm-to-vacuole targeting ; DMSO ; dimethyl sulfoxide ; DTT ; dithiothreitol ; EDTA ; ethylenediamine tetraacetic acid ; ER ; endoplasmic reticulum ; FACS ; fluorescence-activated cell sorting ; GFP ; green fluorescent protein ; HOG ; high osmolarity glycerol ; MOPS ; 3-(N-morpholino)propanesulfonic acid ; PAS ; phagophore assembly site ; PBS ; phosphate buffer saline ; PCR ; polymerase chain reaction ; PDS ; p
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：1861
- 期：1
- 页码：21-33
- 全文大小：1264 K
文摘
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The impact of ceramide signaling on vacuolar function and autophagy was studied.
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Isc1p (sphingomyelinase) deficiency impairs vacuolar structure and functions.
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The isc1Δ cells exhibit defective vesicular trafficking and autophagy.
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These phenotypes are mediated by the ceramide-activated protein phosphatase Sit4p.
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Downregulation of TORC1–Sch9p signaling also suppresses isc1Δ phenotypes.