Bone morphogenetic protein 6 stimulates mineralization in human dental follicle cells without dexamethasone

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• 作者：Kosuke Takahashi^a ; Naomi Ogura^a ; ^b ; Haruna Aonuma^a ; Ko Ito^a ; Daisuke Ishigami^a ; Yoshikazu Kamino^a ; ^b ; Toshirou Kondoh^a ; ^b ; ^{kondo.toshiro@nihon-u.ac.jp}
• 关键词：Human dental follicle cells ; Bone morphogenetic protein-6 ; Dexamethasone ; Osteogenic differentiation ; Stem cells
• 刊名：Archives of Oral Biology
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：58
• 期：6
• 页码：690-698
• 全文大小：923 K

文摘

Objective

The aim of this study is to investigate the osteogenic differentiation human dental follicle cells (hDFCs) cultured with in osteogenic induction medium (OIM) without dexamethasone (DEX), and to analyze the gene expression profile during osteogenic differentiation.

Methods

hDFCs, which isolated from dental follicle tissue from impacted third molar teeth, were cultured with OIM with or without DEX. Osteogenic differentiation of hDFCs was examined using Alkaline phosphatase activity and Arizarin red staining. Gene expression analysis was performed by Microarray and real time-PCR.

Results

We showed that hDFCs have the capacity to differentiate into osteogenic lineages in osteogenic induction medium lacking DEX. We also analyzed gene expression profiling of hDFCs during osteogenic differentiation. BMP6 is up-regulated in both the presence and absence of DEX. In addition, BMP6 enhances gene expression levels of DLX-5, Runx2, and Osterix, which are transcription factors associated with osteogenic differentiation. BMP6 also stimulates phosphorylation of Smad1/5/8 which are transcription factors associated with BMP signalling at protein levels. Additionally BMP6 stimulates mineralization of hDFCs monolayers examined by Arizarin red S staining.

Conclusion

These findings suggest that hDFCs can differentiate to osteogenic lineage cells osteogenic induction medium without DEX, and BMP6 is a key gene in the osteogenic differentiation of hDFCs, and has therapeutic utility for bone regeneration and bone research.