Idiotypic network mimicry and antibody catalysis: lessons for the elicitation of efficient anti-idiotypic protease antibodies
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- 作者：Pillet ; Dominique ; Paon ; Marjorie ; Vorobiev ; Ivan I. ; Gabibov ; Alexander G. ; Thomas ; Daniel ; et. al.
- 关键词：Catalytic antibody ; Abzyme ; Anti-idiotypic catalytic antibody ; Protease activity ; AAPFpNa ; succinyl&ndash ; alanine&ndash ; alanine&ndash ; proline&ndash ; phenylalanine&ndash ; para-nitroanilide ; GGLpNa ; glutaryl&ndash ; glycine&ndash ; glycine&ndash ; leucine&ndash ; par
- 刊名：Journal of Immunological Methods
- 出版年：2002
- 出版时间：November 1, 2002
- 年：2002
- 卷：269
- 期：1-2
- 页码：5-12
- 全文大小：174 K
文摘
An important challenge in the field of catalytic antibodies is the generation of antibodies with designed sequence-specific protease activities. Such catalysts would not only be recruited for diverse applications in basic biological science, but could also offer new approaches in biotechnology and medicine. We have previously used the “internal image” property of the idiotypic network to elicit antibodies with efficient esterase and amidase activities. In the present report, we present preliminary results for the production of anti-idiotypic antibodies mimicking subtilisin. A monoclonal inhibitory antibody of subtilisin was characterized and used to elicit anti-idiotypic antibodies. Some of these antibodies exhibit not only an amidase activity against synthetic substrates, but are also able to cleave a protein, bovine serum albumin (BSA).