Preparation of phosphonated gelatin-coated titanium containing rhBMP-2 by UV irradiation for improved osteoinduction and function

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• 作者：Yun Heo^a ; Eun-Hye Kim^a ; Eiry Kobatake^b ; Jae-Woon Nah^c ; Yoshihiro Ito^d ; Tae-Il Son^a ; ^{tisohn@cau.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Gelatin ; Drug-delivery system ; Osteoinduction ; Titanium surface modification ; Photo-immobilization
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2016
• 出版时间：25 April 2016
• 年：2016
• 卷：36
• 期：Complete
• 页码：66-73
• 全文大小：2070 K

文摘

Gelatin derivative was prepared to coating titanium surface and photo-immobilize rhBMP-2 for improved osteoinduction and function. The conjugation was confirmed by ³¹P and ¹H NMR. Photo-immobilization of the azide–phosphonate–gelatin (Az–P–Gel) was evaluated by micropatterning. The rate of recombinant human bone morphogenetic protein (rhBMP-2) release was evaluated for performance of Az–P–Gel as a carrier. The water contact angle was determined to characterize Ti surface coated with Az–P–Gel. Ability to induce osteogenesis of Az–P–Gel was evaluated using alkaline phosphatase (ALP) activity assay, Alizarin Red S staining, and calcium colorimetric assay. Our results demonstrate that Ti modified with Az–P–Gel and rhBMP-2 facilitates bone regeneration.