Effect of mating history on gender preference in the hermaphroditic snail Physa acuta

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• 作者：Benoî ; t Facon ; Virginie Ravignē ; Laure Sauteur ; Jé ; rô ; me Goudet
• 关键词：freshwater snail ; gender preference ; hermaphrodite ; mating history ; mating system ; Physa acuta ; reproductive behaviour ; sexual conflict
• 刊名：Animal Behaviour
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：74
• 期：5
• 页码：1455-1461
• 全文大小：257 K

文摘

Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables. We here report that SFN is a potent inhibitor of LPS-induced monocyte adhesion, and also blocks the gene expression of the adhesion molecule, ICAM-1, at non-toxic concentrations. Downstream of ICAM-1, NF- κB activity was also found to be abolished in a dose-and time-dependent by SFN in LPS-treated endothelial cells (ECs). SFN exerts its suppressive effects on NF- κB activity in these cells by preventing the degradation of IκB-α. Interestingly, the inhibition of P65 translocation and IκB-α degradation was reversed slightly after 12 hours pretreatment. The intracellular GSH levels in SFN-treated ECs were observed to be reduced, the time course coincident with the suppression of P65 translocation and IκB-α degradation. NAC and GSH reverse the inhibitory effects of SFN upon p65 translocation and IκB-α degradation when preincubated with this agent. Furthermore, the use of BSO to decrease intracellular GSH levels further enhanced the effects of SFN. These data thus suggest that the anti-inflammatory mechanisms of SFN are dependent upon intracellular glutathione level.