Evaluation of anti-proliferative and anti-inflammatory activities of Pelagia noctiluca venom in Lipopolysaccharide/Interferon-γ stimulated RAW264.7 macrophages

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• 作者：Yosra Ayed^a ; ^b ; ^{yosraayed82@gmail.com} ; Rabiaa Manel Sghaier^c ; ^d ; Dhafer Laouini^c ; Hassen Bacha^b ; ^e
• 关键词：F ; fraction ; NO ; nitric oxide ; iNOS ; inducible nitric oxide synthase ; LPS ; lipopolysaccharide ; IFN-γ ; interferon-gamma ; PHA ; phytohemagglutinin ; PBMC ; speripheral blood mononuclear cells ; COX-2 ; cyclooxygenase-2
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：1986-1991
• 全文大小：1080 K
• 卷排序：84

文摘

Components of Pelagia noctiluca (P. noctiluca) venom were evaluated for their anticancer and nitric Oxide (NO) inhibition activities. Three fractions, out of four, obtained by gel filtration on Sephadex G75 of P. noctiluca venom revealed an important selective anti-proliferative activity on several cell lines such as human bladder carcinoma (RT112), human glioblastoma (U87), and human myelogenous leukemia (K562) but not on mitogen-stimulated peripheral blood mononuclear cells. Interestingly, P. noctiluca components showed an important dose-dependent anti-inflammatory activity, through inhibition of NO production via transcriptional regulation of Inducible NO Synthase (iNOS), in IFN-γ/LPS stimulated RAW 264.7 macrophages. These data strongly suggest that P. noctiluca venom could be used as a natural inhibitor of cancer cell lines and a potent anti-inflammatory agent for the treatment of anti-inflammatory diseases.