An automatic optosensing device for the simultaneous determination of resveratrol and piceid in wines

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• 作者：Lucí ; a Molina-Garcí ; a^a ; Antonio Ruiz-Medina^a ; Marí ; a Luisa Ferná ; ndez-de C&#xf3 ; rdova ; ^a ; ^{mferna@ujaen.es}
• 关键词：Resveratrol ; Piceid ; Multicommutation ; Optosensor ; Photochemically induced fluorescence ; Wine
• 刊名：Analytica Chimica Acta
• 出版年：2011
• 出版时间：18 March 2011
• 年：2011
• 卷：689
• 期：2
• 页码：226-233
• 全文大小：523 K

文摘

For the first time, a spectrofluorimetric method is reported for the simultaneous determination of resveratrol (RVT) and piceid (PCD), two stilbenes showing diverse interesting physiological and biochemical attributes, as well as a wide range of health benefits ranging from cardioprotection to chemoprevention. The method makes use of a multicommutated flow-through optosensor in which the resolution of RVT and PCD is accomplished by means the sequential arrival of their photoproducts, on-line generated by UV-irradiation, to the detection area. This is possible due to the different kinetic behaviour of these latter on a solid support (C₁₈ silica gel) filling a minicolumn placed before the detector. The measurement in solid-phase of the photochemically induced fluorescence of the photoproducts (λ_ex: 257 nm/λ_em: 382 nm) is used as analytical signal for monitoring both compounds. The method has been applied to the analysis of RVT and PCD in wines and requires a previous solid-phase extraction (SPE) using Bakerbond C₁₈ cartridges. This pretreatment and the use of a solid-support in both the minicolumn and the flow-cell of the detector allow the determination of RVT and PCD by external calibration. Detection limits (DLs) are 9.3 and 12.6 ng mL⁻¹ for RVT and PCD, respectively. Commercial red and white wine samples have been analysed and the results obtained have been satisfactorily validated by high-performance liquid chromatography (HPLC).