A cluster of novel serotonin receptor 3-like genes on human chromosome 3

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• 作者：Karnovsky ; Alla M. ; Gotow ; Lisa F. ; McKinley ; Denise D. ; Piechan ; Julie L. ; Ruble ; Cara L. ; et. al.
• 关键词：Ligand-gated ion channel ; 5-HT₃ receptor ; Intestine ; Pseudogene ; 5-HT ; 5-hydroxytryptamine ; EST ; expressed sequence tag ; BLAST ; basic local alignment search tool ; CPM ; counts per minute ; RT-PCR ; reverse transcription polymerase chain reaction
• 刊名：Gene
• 出版年：2003
• 出版时间：November 13, 2003
• 年：2003
• 卷：319
• 期：Complete
• 页码：137-148
• 全文大小：2.33 M

文摘

The ligand-gated ion channel family includes receptors for serotonin (5-hydroxytryptamine, 5-HT), acetylcholine, GABA, and glutamate. Drugs targeting subtypes of these receptors have proven useful for the treatment of various neuropsychiatric and neurological disorders. To identify new ligand-gated ion channels as potential therapeutic targets, drafts of human genome sequence were interrogated. Portions of four novel genes homologous to 5-HT_3A and 5-HT_3B receptors were identified within human sequence databases. We named the genes 5-HT_3C1–5-HT_3C4. Radiation hybrid (RH) mapping localized these genes to chromosome 3q27–28. All four genes shared similar intron–exon organizations and predicted protein secondary structure with 5-HT_3A and 5-HT_3B. Orthologous genes were detected by Southern blotting in several species including dog, cow, and chicken, but not in rodents, suggesting that these novel genes are not present in rodents or are very poorly conserved. Two of the novel genes are predicted to be pseudogenes, but two other genes are transcribed and spliced to form appropriate open reading frames. The 5-HT_3C1 transcript is expressed almost exclusively in small intestine and colon, suggesting a possible role in the serotonin-responsiveness of the gut.