Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils

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• 作者：Pierre-Alain Maron ; Magali Maitre ; Anne Mercier ; David Pierre Henri Lejon ; Virginie Nowak ; Lionel Ranjard
• 关键词：Soil microbiology ; Bacterial community ; Metaproteome ; Protein fingerprinting ; DNA fingerprinting
• 刊名：Research in Microbiology
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：159
• 期：4
• 页码：231-236
• 全文大小：422 K

文摘

The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and soil parameters, with pH, clay and CaCO₃ contents being the most discriminating factors.