- 作者：M.D. Pinazo-Durá ; n<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>1sup><sup> ; sup><sup>3sup><sup> ; sup><sup>dolores.pinazo@uv.es" class="auth_mail" title="E-mail the corresponding authorsup> ; V. Zanó ; n-Moreno<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>2sup><sup> ; sup><sup>3sup> ; A. Lleó ; -Perez<sup>asup> ; J.J. Garcí ; a-Medina<sup>asup><sup> ; sup><sup>csup><sup> ; sup><sup>1sup> ; C. Galbis-Estrada<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>1sup> ; M.J. Roig-Revert<sup>asup> ; C. Marco-Ramí ; rez<sup>asup> ; M. Ló ; pez-Gá ; lvez<sup>dsup><sup> ; sup><sup>1sup> ; R. Dolz-Marco<sup>esup><sup> ; sup><sup>1sup> ; L. Duarte<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>csup><sup> ; sup><sup>dsup><sup> ; sup><sup>esup> ; C. Campos Borges<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>csup><sup> ; sup><sup>dsup><sup> ; sup><sup>esup> ; J. Salgado-Borges<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>csup><sup> ; sup><sup>dsup><sup> ; sup><sup>esup> ; R. Gallego-Pinazo<sup>esup><sup> ; sup><sup>1sup>
- 关键词：Interference by RNA ; Micro RNAs ; Type 2 diabetes mellitus ; Diabetic retinopathy ; Pre-clinical biomarkers
- 刊名：Archivos de la Sociedad Española de Oftalmología (English Edition)
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：91
- 期：5
- 页码：209-216
- 全文大小：1198 K
sSec_2">Material and methods
spar0010">A prospective multicentre case–control study of 132 participants was distributed into: T2D with (+DR) or without (−DR) (T2DG; n = 77), and a control group (CG; n = 55). After an eye examination and personal interview, tears were collected for molecular analysis (expression of microRNAs [miRNAs] (miRCURY™ ARN Isolation Kit, Qiagen)]. Libraries, 137 vs. 140 bp (GeneMapper, Applied Biosystems), were obtained in 18 samples (T2DG + DR = 6; T2DG − DR = 6; CG = 6) by performing next-generation sequencing (NGS). SPSS 15.0 statistical program was used to perform data analysis.
sSec_3">Results
spar0015">The mean age was 67 ± 12 years in the T2DG vs. 55 ± 21 years in the CG. Distribution men/women: 25/30 in T2DG vs. 51/28 in CG. A family history of DM, diet compliance, smoking, drinking and exercise, showed significant differences between groups (p < 0.001). A 20–25 μL sample of tears contained a mean of 9.42 ± 3.30 ng/mL of purified ARN, with significant differences between T2DG/CG (p = 0.002) and T2DG + RD/CG (p = 0.004). Tear expression of miARNs in T2DG directly correlated with age/obesity/T2D duration (p < 0.05), and indirectly with visual acuity (p < 0.05). A total of 14 miRNAs related to the presence, pathogenic mechanisms and risk factors for the progression of diabetic retinopathy, were identified.
sSec_4">Conclusions
spar0020">We propose to use tears as a source of genetic information for DM. Specific miRNAs involved in DR development and/or progression can be used as molecular biomarkers, and based on these, for developing future biotherapies.