Designing of a novel dextransucrase efficient in synthesizing oligosaccharides

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• 作者：Qiu-Ping Li ; Chao Wang ; Hong-Bin Zhang ; ^{hbzhang@hfut.edu.cn} ; Xue-Qin Hu ; Ruo-Han Li ; Jing-Han Hua
• 关键词：Dextransucrase ; Oligosaccharide ; Transglycosylation
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：696-703
• 全文大小：1393 K
• 卷排序：95

文摘

The mutant DSR-S1-ΔA was constructed by deleting 1494 bp fragment of DSR-dex-YG. DSR-S1-ΔA enzyme heavily impaired polysaccharide synthesis ability. DSR-S1-ΔA produced more oligosaccharides of lower DP with different acceptors. DSR-S1-ΔA improved the selection range of acceptors to acceptor response. It has a great contribution and research value to the transglycosylation.