Roles of translesion synthesis DNA polymerases in the potent mutagenicity of tobacco-specific nitrosamine-derived O2-alkylthymidines in human cells
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- 作者：Savithri Weerasooriyaa ; 1 ; Vijay P. Jastia ; 1 ; 2 ; Arindam Bosea ; Thomas E. Sprattb ; Ashis K. Basua ; ashis.basu@uconn.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：NNK ; 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone ; NNN ; N'-nitrosonornicotine ; O2-Me-dT ; O2-methylthymidine ; O2-POB-dT ; O2-[4-(3-pyridyl)-4-oxobut-1-yl]thymidine ; HEK293T ; human embryonic kidney 293T ; TLS ; translesion synthesis ; MF ; mutation frequency ; pol ; DNA polymerase ; Kf- ; exo-free Klenow fragment of DNA polymerase I ; Dpo4 ; Sulfolobus solfactaricus DNA polymerase IV
- 刊名：DNA Repair
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：35
- 期：Complete
- 页码：63-70
- 全文大小：490 K
文摘
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O2-Me-dT and O2-POB-dT are replication blocking and highly mutagenic lesions in human cells
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Both O2-Me-dT and O2-POB-dT induce T → A as the major type of mutations in human cells.
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Pols η, ζ and Rev1 play important roles in the TLS of O2-Me-dT and O2-POB-dT.
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Pol ζ is a key enzyme for mutagenesis induced by O2-POB-dT.