Immobilizing and stretching of poly(dG)–poly(dC) synthesized by Klenow fragment exo⁻ on substrates by LB method

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• 作者：Aya Tanaka ; Yasutaka Matsuo and Kuniharu Ijiro
• 关键词：DNA ; Poly(dG)– ; poly(dC) ; Langmuir– ; Blodgett method ; Polyion complex monolayer ; DNA polymerase
• 刊名：Colloids and Surfaces A: Physicochemical and Engineering Aspects
• 出版年：2006
• 出版时间：15 August 2006
• 年：2006
• 卷：284-285
• 期：Complete
• 页码：246-249
• 全文大小：338 K

文摘

We synthesized poly(dG)–poly(dC) by the enzymatic extension of dG₁₀–dC₁₀ with Klenow fragment exo⁻. The length of prepared poly(dG)–poly(dC) was determined by the electrophoresis and the Langmuir–Blodgett (LB) method. Cationic amphiphile (2C₁₈N⁺2C₁) was spread on an aqueous solution of synthesized poly(dG)–poly(dC). And a formed polyion complex monolayer at the air–water interface due to the electrostatic interaction between the amphiphile and poly(dG)–poly(dC) was transferred on a glass substrate. The fluorescence images of the transferred polyion complex monolayers showed that single poly(dG)–poly(dC) molecules were stretched on the glass substrate and the lengths of the stretched molecules almost corresponded to 21,000 bp. The stretched poly(dG)–poly(dC) can be used as a sample for electric conductivity measurement and a template for spatially arranging molecules and metals.