Surface modification of a titanium alloy with a phospholipid polymer prepared by a plasma-induced grafting technique to improve surface thromboresistance

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• 作者：Sang Ho Ye ; Carl A. Johnson Jr ; Joshua R. Woolley ; Heung-Il Oh ; Lara J. Gamble ; Kazuhiko Ishihara ; William R. Wagner
• 关键词：Titanium alloy ; Surface modification ; Phospholipid polymer ; Blood compatibility ; Ventricular assist device (VAD)
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2009
• 出版时间：1 November 2009
• 年：2009
• 卷：74
• 期：1
• 页码：96-102
• 全文大小：934 K

文摘

To improve the thromboresistance of a titanium alloy (TiAl₆V₄) surface which is currently utilized in several ventricular assist devices (VADs), a plasma-induced graft polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) was carried out and poly(MPC) (PMPC) chains were covalently attached onto a TiAl₆V₄ surface by a plasma induced technique. Cleaned TiAl₆V₄ surfaces were pretreated with H₂O-vapor-plasma and silanated with 3-methacryloylpropyltrimethoxysilane (MPS). Next, a plasma-induced graft polymerization with MPC was performed after the surfaces were pretreated with Ar plasma. Surface compositions were verified by X-ray photoelectron spectroscopy (XPS). In vitro blood biocompatibility was evaluated by contacting the modified surfaces with ovine blood under continuous mixing. Bulk phase platelet activation was quantified by flow cytometric analysis, and surfaces were observed with scanning electron microscopy after blood contact. XPS data demonstrated successful modification of the TiAl₆V₄ surfaces with PMPC as evidenced by increased N and P on modified surfaces. Platelet deposition was markedly reduced on the PMPC grafted surfaces and platelet activation in blood that contacted the PMPC-grafted samples was significantly reduced relative to the unmodified TiAl₆V₄ and polystyrene control surfaces. Durability studies under continuously mixed water suggested no change in surface modification over a 1-month period. This modification strategy shows promise for further investigation as a means to reduce the thromboembolic risk associated with the metallic blood-contacting surfaces of VADs and other cardiovascular devices under development.