A phytochemical investigation of secondary metabolites from Micromelum integerrimum was reported. Two new prenylated coumarins named as hydramicromelin D (rong class="boldFont">1rong>) and integerrimelin (rong class="boldFont">2rong>) were isolated along with 9 known coumarin derivatives. The chemical structures as well as relative stereochemistries of those coumarins were confirmed on the basis of extensive spectroscopic data interpretation and comparison with those previously published data.