Molecular recognition of Cullin3 by KCTDs: Insights from experimental and computational investigations
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- 作者：Nicole Balascoa ; b ; Luciano Pironec ; Giovanni Smaldonea ; Sonia Di Gaetanoa ; Luciana Espositoa ; Emilia Maria Pedonea ; Luigi Vitaglianoa ; luigi.vitagliano@unina.it" class="auth_mail
- 关键词：MD ; molecular dynamics ; RMSF ; root mean square fluctuation ; RMSD ; root mean square deviation ; RMSIP ; root mean square inner product ; ITC ; isothermal titration calorimetry ; PDB ; Protein Data Bank ; INC ; insomniac protein ; Cul3 ; Cullin3 ; CRL ; Cullin RING ligase
- 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
- 出版年：July 2014
- 年：2014
- 卷：1844
- 期：7
- 页码：1289-1298
- 全文大小：2439 K
文摘
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We report the first quantification of Cul3 binding to a protein of the KCTD family.
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MD simulations highlight the regions involved in Cul3-KCTD interactions.
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KCTD5-Cul3 recognition is characterized by a high level structural complexity.
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Homology modeling indicates that INC interacts with Cul3 using a similar mechanism.
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The architecture of the entire KCTD5-containing E3 ligase system has been analyzed.