文摘
In human pancreatic adenocarcinoma BxPC3 cells, high mPR mRNA levels and the absence of nPR mRNA have been detected. Western blot analysis showed the presence of the mPRα, mPRβ, mPRγ proteins and the absence of nPR protein in BxPC3 cells. In BxPC3 cells, progesterone at high concentration of 20 mM significantly reduced the mRNA levels of proliferation markers Ki67 and PCNA, as well as of cyclin D1. In BxPC3 cells, progesterone at high concentration of 20 μM significantly increased the mRNA levels of cyclin dependent kinase inhibitors p21 and p27. Progesterone at 1 μM and 20 μM concentrations inhibited proliferative activity of BxPC3 cells.