A teleostean angiotensinogen from Oplegnathus fasciatus responses to immune and injury challenges

详细信息    查看全文

• 作者：Navaneethaiyer Umasuthan^a ; Ilson Whang^a ; Kasthuri Saranya Revathy^a ; Myung-Joo Oh^b ; Sung-Ju Jung^b ; Cheol Young Choi^c ; Jeong-Ho Lee^d ; Jae Koo Noh^d ; Jehee Lee^a ; ^e ; ^{jehee@jejunu.ac.kr}
• 关键词：Angiotensinogen ; Serpin ; Rock bream ; Injury ; TNF-¦Á
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：32
• 期：5
• 页码：922-928
• 全文大小：939 K

文摘

Angiotensinogen (AGT) is the precursor of the renin-angiotensin system and contributes to osmoregulation, acute-phase and immune responses. A full-length cDNA of the AGT (2004?bp with a 1389?bp coding region) was isolated from rock bream (Rb), Oplegnathus fasciatus. The encoded polypeptide of 463 amino acids had a predicted molecular mass of 51.6?kDa. RbAGT possessed a deduced signal peptide of 22 residues upstream of a putative angiotensin I sequence (²³NRVYVHPFHL³²). RbAGT possessed a specific domain profile and a signature motif which are characteristics of the serpin family. Sequence homology and phylogenetic analysis indicated that RbAGT was evolutionarily closest to AGT of Rhabdosargus sarba. The mRNA expression profile of RbAGT was determined by quantitative RT-PCR and it demonstrated a constitutive and tissue-specific expression with the highest transcript level in the liver. Significantly up-regulated RbAGT expression was elicited by systemic injection of a lipopolysaccharide, rock bream iridovirus (RBIV) and bacteria (Edwardsiella tarda and Streptococcus iniae), revealing its pathogen inducibility. RbAGT manifested a down-regulated response to systemic injury, contemporaneously with two other serpins, protease nexin-1 (PN-1), and heparin cofactor II (HCII). In addition, a synchronized expression pattern was elicited by RbAGT and RbTNF-¦Á in response to injury, suggesting that TNF-¦Á might be a potential modulator of AGT transcription.