Sequences in Linker-1 domain of the multidrug resistance associated protein (MRP1 or ABCC1) bind to tubulin and their binding is modulated by phosphorylation

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• 作者：Raghuram Ambadipudi ; Elias Georges ; ^{elias.georges@mcgill.ca}
• 关键词：MRP1 ; ABCC1 ; Protein interactions ; Tubulin ; Linker 1 domain ; Phosphorylation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1001-1006
• 全文大小：878 K
• 卷排序：482

文摘

This study shows direct binding between MRP1 Linker 1 (L1MRP1) domain and α- and β-tubulin subunits, using a scanning peptide approach. Four short and dispersed sequences in L1MRP1 domain interact with α/β-tubulin subunits. Each sequence in L1MRP1-tubulin binding domain contains predicted protein kinase C/A site. Substituting 3 of 4 serine with aspartic acid, but not alanine, in L1MRP1 binding domains inhibit their binding to tubulin subunits.