Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase, a nitrogenase-like enzyme, from Rhodobacter capsulatus

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• 作者：Jiro Nomata ; Toru Kondo ; Shigeru Itoh ; Yuichi Fujita
• 关键词：Bchl ; bacteriochlorophyll ; Chl ; chlorophyll ; Chlide ; chlorophyllide a ; DPOR ; dark-operative protochlorophyllide oxidoreductase ; LPOR ; light-dependent protochlorophyllide oxidoreductase ; NA ; nicotinamide ; Pchlide ; protochlorophyllide
• 刊名：FEBS Letters
• 出版年：2013
• 出版时间：17 September, 2013
• 年：2013
• 卷：587
• 期：18
• 页码：3142-3147
• 全文大小：1501 K

文摘

Dark-operative protochlorophyllide oxidoreductase (DPOR) is a nitrogenase-like enzyme consisting of two components, L-protein as a reductase component and NB-protein as a catalytic component. Elucidation of the crystal structures of NB-protein (Muraki et al., Nature 2010, 465: 110-114) has enabled us to study its reaction mechanism in combination with biochemical analysis. Here we demonstrate that nicotinamide (NA) inhibits DPOR activity by blocking the electron transfer from L-protein to NB-protein. A reaction scheme of DPOR, in which the binding of protochlorophyllide (Pchlide) to the NB-protein precedes the electron transfer from the L-protein, is proposed based on the NA effects.