Validation of RT-qPCR reference genes and determination of Robo4 expression levels in human retinal endothelial cells under hypoxia and/or hyperglycemia
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- 作者：Jia'nan Xie ; Xin Liu ; Ying Li ; Yang Liu ; Guanfang Su ; sugf2012@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：RT-qPCR ; real-time reverse transcription quantitative polymerase chain reaction ; HREC ; human retinal endothelial cells ; DR ; diabetic retinopathy ; Robo4 ; roundabout4 ; RPE ; retinal pigment epithelium ; PDR ; proliferative diabetic retinopathy ; 18S ; 18S ribosomal RNA ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; B2M ; beta-2-microglobulin ; ACTB ; actin ; beta ; ALAS1 ; 5&prime ; -aminolevulinate synthase 1 ; GUSB ; glucuronidase ; beta ; HPRT1 ; hypoxanthine phosphoribosyl transferase 1 ; HMBS ; hydroxymethylb
- 刊名：Gene
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：585
- 期：1
- 页码：135-142
- 全文大小：736 K
文摘
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TBP, PUM1, and ALAS1 are the most suitable reference genes for RT-qPCR in HREC under hypoxic and/or hyperglycemic conditions.
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The expression level of Robo4 is changed significantly in HREC under hypoxia and hyperglycemia conditions.
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Robo4 plays different roles in various stages of diabetic retinopathy.