Quantitative profiling of O-glycans by electrospray ionization- and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry after in-gel derivatization with isotope-coded 1-phenyl-3-methyl-5-pyrazolone
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- 作者：Sini&scaron ; a Sić ; Norbert M. Maier1 ; Andreas M. Rizzi ; Andreas.Rizzi@univie.ac.at" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mass spectrometry ; Stable isotope coded labeling ; O-glycans ; 1-Phenyl-3-methyl-5-pyrazolone ; Ionic liquid matrix
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：7 September 2016
- 年：2016
- 卷：935
- 期：Complete
- 页码：187-196
- 全文大小：639 K
文摘
- 10">
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10">Isotope coded labeling of O-glycans by PMP is evaluated as in-gel reaction after 1D-GE (innovative step).
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15">In-gel reaction results in only marginal loss of sensitivity (LOD) compared to in-solution reaction.
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Sensitivity of MALDI-MS is comparable with those attained by RP-HPLC-ESI-MS. Reproducibility and reliability of quantitation is worse.
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5">The applicability of in-gel reaction for analyzing O-glycosylation in proteins of therapeutic relevance is demonstrated.