Hydrolysis of model cellulose films by cellulosomes: Extension of quartz crystal microbalance technique to multienzymatic complexes

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• 作者：Shanshan Zhou^a ; Hsin-Fen Li^a ; Ravinder Garlapalli^a ; Sue E. Nokes^b ; Michael Flythe^c ; ^d ; Stephen E. Rankin^a ; Barbara L. Knutson^a ; ^{bknutson@engr.uky.edu}
• 关键词：Cellulosomes ; QCM-D ; Solid substrate ; Cellobiose inhibition
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：42-49
• 全文大小：2949 K
• 卷排序：241

文摘

QCM measurement of binding and hydrolysis extended to bacterial cellulosomes. Effect of environmental perturbations (cellobiose inhibition) quantified by QCM. Unexpected level of cell-free cellulosome production relevant to bioconversion. Interfacial measurement provides real-time data for kinetic modeling.