Intronic miR-301 feedback regulates its host gene, ska2, in A549 cells by targeting MEOX2 to affect ERK/CREB pathways

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• 作者：Guojun Cao ; Baochun Huang ; Zhaohui Liu ; Jiyan Zhang ; Hua Xu ; Wei Xia ; Jie Li ; Shaohua Li ; Liucun Chen ; Hongmei Ding ; Qiang Zhao ; Ming Fan ; Beifen Shen ; Ningsheng Shao
• 关键词：MicroRNA ; miR-301 ; ska2 ; CREB
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：11 June 2010
• 年：2010
• 卷：396
• 期：4
• 页码：978-982
• 全文大小：829 K

文摘

miR-301 is localized in the first intron of ska2, whose function has not been clarified. Here, a new circuit model in which intronic miR-301 regulates the transcription and function of its host gene through a feedback mechanism has been described. Our results showed that blocking of miR-301 in A549 cells leads to a decrease in the expression of the host gene, ska2. Further analysis showed that miR-301 targets MEOX2 to affect the ERK/CREB pathway. CREB directly regulates the expression of the host gene, ska2. In addition, the inhibition of miR-301 or ska2 resulted in an increase of the mitotic index and a decrease in colony formation in soft agar, which may be related to lung tumorigenesis.