Determination of benzimidazole residues in animal tissue samples by combination of magnetic solid-phase extraction with capillary zone electrophoresis

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• 作者：Xi-Zhou Hu^a ; ^b ; Ming-Luan Chen^a ; Qiang Gao^a ; ^c ; Qiong-Wei Yu^a ; Yu-Qi Feng^a ; ^{yqfeng@whu.edu.cn}
• 关键词：Benzimidazole drugs ; Magnetic solid-phase extraction ; Fe3O4/SiO2/poly (MAA-co-EGDMA) magnetic microspheres ; Capillary zone electrophoresis ; Field-amplified sample stacking
• 刊名：Talanta
• 出版年：2012
• 出版时间：30 January, 2012
• 年：2012
• 卷：89
• 期：Complete
• 页码：335-341
• 全文大小：913 K

文摘

Benzimidazole drugs (BZDs) comprise a large number of synthetic anthelmintics, which are widely used in food-producing animals for prophylactic and therapeutic purposes. To protect consumers from the risks related to BZDs residues, a simple, rapid, and efficient method for simultaneous determination of ten BZDs in animal tissues samples was developed. This analytical procedure involved extracting samples with magnetic solid-phase extraction (MSPE) using magnetite/silica/poly (methacrylic acid-co-ethylene glycol dimethacrylate) (Fe₃O₄/SiO₂/poly (MAA-co-EGDMA)) magnetic microspheres, and determination by capillary zone electrophoresis (CZE). To improve the sensitivity of the method, we employed the electrokinetic injection with field-amplified sample stacking technique (FASS). Berbine solution was used as internal standard to minimize the fluctuation of analytical results. Under the optimized extraction conditions, good linearities were obtained for the ten BZDs with the correlation coefficients (R²) above 0.9920. The limits of detections (LODs) for ten BZDs were 1.05-10.42 ng/g in swine muscle and 1.06-12.61 ng/g in swine liver, respectively. The intra- and inter-day relative standard deviations (RSDs) of the developed method were less than 13.6 % . The recoveries of the ten BZDs for the spiked samples ranged from 81.1 % to 105.4 % with RSDs less than 9.3 % .