Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris

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• 作者：Lei Wang ; Chun-e Lai ; Qifeng Wu ; Junliang Liu ; Maojun Zhou ; Zhenghua Ren ; D ; an Sun ; Shangwu Chen ; Anlong Xu
• 关键词：Antimicrobial peptide ; HKABF ; Recombinant expression ; Purification ; Pichia pastoris ; Antimicrobial activity
• 刊名：Process Biochemistry
• 出版年：2008
• 出版时间：October 2008
• 年：2008
• 卷：43
• 期：10
• 页码：1124-1131
• 全文大小：877 K

文摘

HKABF, Hippocampus kuda antibacterial factor, is a putative antimicrobial peptide identified from the cDNA library of the brooding pouch of H. kuda Bleeker. The putative mature peptide, fused with a 6His-tag, an anionic peptide (SAPLNPDNID) and to an enterokinase cleavage site was expressed inPichia pastoris. The mature HKABF peptide, released by enterokinase cleavage, showed antimicrobial activity against Staphylococcus aureus and Staphylococcus saprophyticus at low concentrations (0.5–2.5 μg/ml). The recombinant mature peptide was heat-stable and had low hemolytic activity. Circular dichroism (CD) spectroscopy data indicates its classification as a CSαβ-type antimicrobial peptide. This study serves as an impetus for the production and further research of this antimicrobial peptide.