A novel therapeutic vaccine of GM-CSF/TNFα surface-modified RM-1 cells against the orthotopic prostatic cancer

详细信息    查看全文

• 作者：Weihua Yin ; Qiushan He ; Zhiming Hu ; Zhong Chen ; Mao Qifeng ; Song Zhichun ; Qu Zhihui ; Nie Xiaoxia ; Jinlong Li ; Jimin Gao
• 关键词：GM-CSF ; TNFα ; Therapeutic vaccine ; Prostate cancer
• 刊名：Vaccine
• 出版年：2010
• 出版时间：12 July 2010
• 年：2010
• 卷：28
• 期：31
• 页码：4937-4944
• 全文大小：650 K

文摘

A novel therapeutic vaccine against prostate cancer was developed by simultaneous immobilization of streptavidin-tagged bioactive GM-CSF and TNFα on the biotinylated surface of 30 % ethanol-fixed RM-1 prostatic cancer cells. This study showed that the GM-CSF/TNFα-doubly surface-modified vaccine significantly extended the survival in the orthotopic model of RM-1 prostate cancer, and was superior to single GM-CSF- or TNFα-surface-modified vaccine. Moreover, the splenocytes from the GM-CSF/TNFα-vaccine-treated mice showed the most potent cytotoxicity on RM-1 cells and the highest production of RM-1-specific IFNγ. In addition, more CD4⁺ and CD8⁺ T cells infiltrated into the tumor sites in the GM-CSF/TNFα-vaccine-treated mice than in the GM-CSF- or TNFα-vaccine-treated mice. Therefore, our study demonstrated that the efficacy of RM-1 prostate cancer cell vaccine could be improved by conjugating both GM-CSF and TNFα simultaneously on the surface of cancer cells, and that this modification thus has a potential translational significance.