Quantitative Ultrasound Assessment of Cartilage Degeneration in Ovariectomized Rats with Low Estrogen Levels
详细信息 查看全文
- 作者：Qing Wang&lowast ; ; &dagger ; ; wq8740@smu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhiwei Liu&lowast ; ; Yinong Wang&lowast ; ; Qingya Pan&lowast ; ; Qianjin Feng&lowast ; ; &dagger ; ; qianjinfeng08@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Qinghua Huang&Dagger ; ; Wufan Chen&lowast ; ; &dagger ;
- 关键词：Cartilage degradation ; High-frequency ultrasound ; Ovariectomized rat ; Estrogen ; Post-menopause
- 刊名：Ultrasound in Medicine & Biology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：42
- 期：1
- 页码：290-298
- 全文大小：1102 K
文摘
The aim of this study was to assess quantitatively the site-specific degeneration of articular cartilage in ovariectomized rats with low estrogen levels using a high-frequency ultrasound system. Fourteen female Sprague–Dawley rats were randomly divided into two groups (n = 7 per group): a sham group in which only the peri-ovarian fatty tissue was exteriorized and an ovariectomized group that underwent bilateral ovariectomy to create a menopause model with low estrogen levels. All animals were sacrificed at the end of the third week after ovariectomy. Hindlimbs were harvested. The articular cartilage from five anatomic sites (i.e., femoral caput [FC], medial femoral condyle [MFC], lateral femoral condyle [LFC], medial tibial plateau [MTP] and lateral tibial plateau [LTP]) was examined with ultrasound. Four parameters were extracted from the ultrasound radiofrequency data: reflection coefficient of the cartilage surface (RC1), reflection coefficient of the cartilage–bone interface (RC2), ultrasound roughness index (URI) and thickness of the cartilage tissue. The results indicated significant (p < 0.05) site dependence for cartilage thickness, URI and RC1 in the sham group. The 3-wk post-menopause ovariectomized rats exhibited significant increases (p < 0.05) in the URI at the LFC, MTP and LTP; significant decreases (p < 0.05) in RC1 at the FC, LFC and MTP; and significant decreases (p < 0.05) in cartilage thickness at the MFC, LFC, MTP and LTP. These results of this study suggest that post-menopausal estrogen reduction induces morphologic and acoustic alterations in the articular cartilage of the hip and knee joints in ovariectomized rats.