Novel T7-like expression systems used for Halomonas

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• 作者：Han Zhao^a ; ¹ ; Haoqian M. Zhang^b ; ^c ; ¹ ; Xiangbin Chen^a ; Teng Li^a ; ^c ; Qiong Wu^a ; Qi Ouyang^b ; ^{qi@pku.edu.cn} ; Guo-Qiang Chen^a ; ^{chengq@mail.tsinghua.edu.cn}
• 关键词：Part mining ; Expression system ; Morphological engineering ; PHB ; Synthetic biology ; Non-model microorganism
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：128-140
• 全文大小：1892 K
• 卷排序：39

文摘

Novel T7-like expression systems were developed for non-model bacteria including Halomonas and Pseudomonas entomophila. The expression systems displayed low crosstalk, tight regulation and high efficiency. Cross-species transferring of pathway control was enabled by the T7-like systems. Ultrahigh PHB accumulation (92% of CDW) was achieved in Halomonas TD01 using T7-like MmP1.