QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism

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• 作者：Can M. Ü ; nal^a ; Birendra Singh^a ; Christophe Fleury^a ; Kalpana Singh^a ; Luis Chá ; vez de Paz^b ; Gunnel Svensä ; ter^b ; Kristian Riesbeck^a ; ^{kristian.riesbeck@med.lu.se}
• 关键词：Biofilm ; NTHi ; Quorum sensing ; Signalling
• 刊名：International Journal of Medical Microbiology
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：302
• 期：6
• 页码：261-269
• 全文大小：1621 K

文摘

Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal violet staining. In addition, under constant flow conditions, Hi3655KR2 formed less biofilm after 48 h. The biofilm defects were irrespective of autoinducer-2 levels. Hence, here we suggest for the first time a regulatory circuit in NTHi, which controls biofilm formation by mechanisms other than or in addition to luxS-dependent factors.