Morphohistological analysis of the origin and development of Rosa canina protocorm-like bodies

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• 作者：Feng-Luan Liu ; Isak Abdurazak ; Lin Xi ; Bin Gao ; Ling Wang ; Chuan-Wei Tian ; Liang-Jun Zhao
• 关键词：2 ; 4-D ; 2 ; 4-dichlorophenoxyacetic acid ; CPDs ; cells in periclinal division ; DAT ; days after transfer of callus rhizoids to thidiazuron medium ; PLB ; protocorm-like body ; PVE ; pitted vessel element ; QC ; quiescent center ; TDZ ; thidiazuron
• 刊名：Scientia Horticulturae
• 出版年：6 March, 2014
• 年：2014
• 卷：167
• 期：Complete
• 页码：107-116
• 全文大小：8769 K

文摘

A protocorm-like bodies (PLBs) regeneration system has been established for several Rosa spp. Greater understanding of the PLB formation process, in particular how PLBs are triggered from the rhizoid meristem, would contribute to the establishment of PLB protocols for cut roses that could be applied for rapid propagation and molecular breeding. The present study investigated the morphogenesis of Rosa canina PLBs. Callus rhizoids of R. canina were cultured on thidiazuron medium to induce PLBs, and the developmental process was examined by stereoscope, scanning electron microscopy, and transmission electron microscopy via paraffin sections and ultrathin sections. The results showed that 5 days after callus rhizoids were transferred to thidiazuron medium (DAT), vascular tissue comprising phloem and xylem was formed from the leaf callus to the rhizoid meristem, where a group of cells dividing periclinally emerged at 9 DAT. In the rhizoid tip, the division and enlargement of those dividing cells induced the formation of starch-rich parenchyma cells, and the totipotent cells adjacent to the epidermis differentiated into meristematic centers at about 20 DAT. PLBs were formed from meristematic centers through globular embryos at approximately 35 DAT, and then germinated precociously without going through the heart, torpedo, and cotyledon stages. This research suggests that R. canina PLBs were derived from parenchyma cells adjacent to the epidermis of the rhizoid tip. Vascular tissue of callus rhizoid was essential for the formation of parenchyma cells in the rhizoid meristem.