High temperature tension and low cycle fatigue experiments of IN718 alloy have been performed in the electro-hydraulic servo system with scanning electron microscope at 455 掳C. Fatigue crack initiation and propagation process are investigated in situ. Results show that the carbide and twin grain are the crack source of the low cycle fatigue of IN718 alloy, and the low cycle fatigue life of the alloy increases with the decrease in grain size.