Screening for enterotoxigenic Bacteroides fragilis in stool samples
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- 作者：Jacqueline I. Keenana ; jacqui.keenan@otago.ac.nz" class="auth_mail" title="E-mail the corresponding author ; Alan Aitchisona ; Rachel V. Purcella ; Rosie Greenleesb ; John F. Pearsonc ; Frank A. Frizellea
- 关键词：Enterotoxigenic Bacteroides fragilis ; Colorectal cancer ; bft gene ; PCR ; Stool samples
- 刊名：Anaerobe
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：40
- 期：Complete
- 页码：50-53
- 全文大小：299 K
文摘
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Molecular identification of enterotoxigenic Bacteroides fragilis (ETBF) in stool samples.
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Comparison of different methods of DNA preparation and ETBF amplification.
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Selective enrichment of Bacteroides increases identification of ETBF.
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Quantitative PCR increases sensitivity over conventional PCR.
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Use of multiple methods offers greatest detection rates.